|本期目录/Table of Contents|

[1]谢芳钦,杨劲松,罗朝晨,等.福建省脑膜炎奈瑟氏菌病原学及分子分型研究[J].慢性病学杂志,2015,(6):609-611.
　XIE Fang-qin,YANG Jin-song,LUO Chao-chen,et al.Study on pathogen and molecular subtyping of Neisseria meningitides in Fujian Province[J].,2015,(6):609-611.
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福建省脑膜炎奈瑟氏菌病原学及分子分型研究(PDF)

《慢性病学杂志》[ISSN:1674-8166/CN:11-5900/R]

卷:
期数:
2015年6期
页码:
609-611
栏目:
论　著
出版日期:
2015-12-27

文章信息/Info

Title:
Study on pathogen and molecular subtyping of Neisseria meningitides in Fujian Province
作者:
谢芳钦;  杨劲松;  罗朝晨;  李曲文;  林震宇;  陈爱平
福建省疾病预防控制中心，福州350001
Author(s):
XIE Fang-qin;  YANG Jin-song;  LUO Chao-chen;  LI Qu-wen;  LIN Zhen-yu;  CHEN Ai-ping
Fujian Provincial Center for Disease Control and Prevention, Fuzhou, Fujian 350001, China Corresponding author: CHEN Ai-ping, E-mail: cap2006@live.cn
关键词:
脑膜炎奈瑟氏菌; 脉冲场凝胶电泳; 菌群分布.
Keywords:
Neisseria meningitides;  Pulsed-field gel electrophoresis;  Serogroups distribution
分类号:
R515；R183
DOI:
-
摘要:
目的对福建省2006-2010 年脑膜炎奈瑟氏菌进行分析，了解菌群的分布、变迁及分子分型特征。 方法对样本进行细菌培养、表型鉴定和脉冲场凝胶电泳（PFGE） 试验。结果15 株流脑样本中血清群 构成比分别为A 群73.33% （11/15）， B 群13.33% （2/15）， C 群6.67% （1/15）， W135 群6.67% （1/15）。 PFGE 图谱及聚类分析发现，相同血清群分离菌株的PFGE 酶切图谱相似度较高，不同血清群的脑膜炎奈 瑟氏菌图谱呈多态性。结论PFGE 能够对脑膜炎奈瑟氏菌鉴定，福建省流行性脑脊髓膜炎流行菌群正发 生变迁，但目前仍以A 群为主，暂无优势型别。同时近年来先后出现B、C、W135 血清群并存的现象，应 密切关注，以防流脑的暴发或者流行。
Abstract:
Objective To understand the serogroup distribution, evolution and molecular subtyping by analyzing the pathogens of Neisseria meningitides (Nm) in Fujian Province from 2006 to 2010. Methods The bacterial specimens of Nm were examined in bacterial culture, phenotypical analysis and pulsedfield gel electrophoresis (PFGE). Results Among the 15 strains of Nm examined, serogroup A was 73.33% (11/15), serogroup B 13.33% (2/15), serogroup C 6.67% (1/15), and serogroup W135 6.67% (1/ 15). Bacterial strains of the same serogroups were relatively similar based on the PFGE enzyme digestion mapping and clustering analysis, while strains of different serogroups appeared diversified in the PFGE mapping. Conclusions PFGE can be used for typing of bacterial strains of Nm. Our analyses provide evidence for the dynamic changes of Nm population in Fujian Province in which no single dominant serogroup exists while serogroup A was the most prevalent serogroup coexisting with serogroup B, C and W135. Our findings suggest that surveillance on serogroup epidemic and outbreaks should be monitored closely, in order to prevent outbreak or epidemic meningitis.

参考文献/References:

[1] 迮文远,刁连东,徐爱强,等.计划免疫学[M].2 版.上海:上海科学 技术文献出版社,2001:461-475. [/br]［2］ Robbins JB, Schneerson R, Gotschlich EC, et al. Meningococ? cal meningitis in sub- Saharan Africa: the case for mass and routine vaccination with available polysaccharide vaccines [J]. Bull World Health Organ, 2003,81(10):745-750. [/br]［3］ 罗朝晨,谢一俊,黄文龙,等.福建省12 年间流行性脑脊髓膜炎 流行病学特征分析[J].现代预防医学,2003,6(30):863-864. [/br]［4］ Popovic T, Schmink S, Rosenstein NA, et al. Evaluation of pulsed- field gel electrophoresis in epidemiological investiga? tions of meningococcal disease outbreaks caused by Neisseria meningitidis Serogroup C [J]. J Clin Microbiol, 2001,39(1): 75-85. [/br]［5］ 胡绪敬.流行性脑脊髓膜炎流行的监测与预防[J].中国公共卫 生,2004,20(5):638-640. [/br]［6］ 原灵,陈爱平,郭维植,等.从一例脑膜炎患者的脑脊液中检出B 群脑膜炎球菌[J].中国预防医学杂志,2008,9(7):663-664. [/br]［7］ 原灵,郭维植,谢一俊,等.从2 例脑膜炎患者的脑脊液中首次检 出C 群脑膜炎球菌[J].中国预防医学杂志,2006,7(2):133-135. [/br]［8］ 原灵,詹銮峰,林震宇.福建省首例W135 群流行性脑脊髓膜炎病 例的回顾性研究[J].疾病监测,2010,3(3):195-196. [/br]［9］ Swaminathan B, Barrett TJ, Hunter SB, et al. PulseNet: The molecular subtyping network for foodborne bacterial disease surveillance, United States [J]. Emerg Infect Dis, 2001,7(3): 382-389. [/br]［10］ 徐丽.我国流行性脑脊髓膜炎的发病形势与预防[J].疾病监测, 2002,17(2):74. [/br]［11］ Ambur OH, Frye SA, Tnjum T. New functional identity for the DNA uptake sequence in transformation and its presence in transcriptional terminators [J]. J Bacteriol, 2007,189(5):2077- 2085.

备注/Memo

备注/Memo:
基金项目：福建省卫生厅青年课题（2012-2-22）；福建 省疾病预防控制中心青年科研课题（2011-03） 作者简介：谢芳钦，硕士，主管医师，研究方向：分子 病原学和分子流行病学 通信作者：陈爱平，E-mail: cap2006@live.cn
更新日期/Last Update: 2015-12-27