|本期目录/Table of Contents|

[1]杨琳,郝民,王恒伟,等.北京市朝阳区早孕人群B群链球菌耐药性及毒力基因分析[J].慢性病学杂志,2019,20(06):802-806.
　YANGLin *,HAOMin,WANGHeng-wei,et al.Analysis of drug resistance and virulencegenes of group B streptococcus in early pregnancy group in Chaoyang district, Beijing[J].,2019,20(06):802-806.
点击复制

北京市朝阳区早孕人群B群链球菌耐药性及毒力基因分析(PDF)

《慢性病学杂志》[ISSN:1674-8166/CN:11-5900/R]

卷:
20
期数:
2019年06期
页码:
802-806
栏目:
论　著
出版日期:
2019-06-28

文章信息/Info

Title:
Analysis of drug resistance and virulencegenes of group B streptococcus in early pregnancy group in Chaoyang district, Beijing
作者:
杨琳1 ; 郝民2 ; 王恒伟2 ; 邵希凤2 ; 宋衍燕2
1.北京市朝阳区妇幼保健院，北京 100021； 2.北京市朝阳区疾病预防控制中心，北京 100021
Author(s):
YANGLin * ; HAOMin; WANGHeng-wei; SHAOXi-feng; SONGYan-yan
*BeijingChaoyang DistrictMaternalandChild Health Hospital,Beijing100021,China Correspondingauthor:SONGYan-yan,E-mail:songyanyan@cycdpc.org
关键词:
B群链球菌; 耐药性; 耐药基因; 毒力基因
Keywords:
Group B streptococcus;  Drugresistance;  Drugresistance genes;  Virulence gene
分类号:
R969.3
DOI:
-
摘要:
目的 了解北京市朝阳区B群链球菌（GBS）耐药性及耐药基因和毒力基因的携带情况。方法 选取分 离于北京市朝阳区妇幼保健院2016年2—6月孕4~10周女性宫颈携带的29株GBS，使用微量肉汤稀释法和药敏 纸片琼脂扩散法（Kirby-Bauer法，K-B法）测定细菌耐药表型，PCR检测菌株耐药基因和毒力基因。结果 29株GBS对青霉素、美罗培南、头孢噻肟、万古霉素敏感率均为100.00%；对红霉素、四环素、克林霉素、左 氧氟沙星、加替沙星、氯霉素、利奈唑胺耐药率依次为82.76%、75.86%、44.83%、37.93%、37.93%、6.90%、 6.90%；29株GBS中检出含有耐药基因pbp1a 28株（96.55%）、pbp2b 26株（89.66%）、gyrB 19株 （65.52%）、linB15株（51.72%）、ermB14株（48.28%）、tetM13株（44.83%）、mefA11株（37.93%）、 tetK8株（27.59%）、tetO5株（17.24%）；检出含有毒力基因lmb 22株（75.86%）、ScpB10株（34.48%）、 bca 13株（44.83%）、hylB10株（34.48%）、bac 8株（27.59%）。结论 北京市朝阳区GBS对青霉素尚未出现 耐药菌株，针对青霉素过敏患者不建议使用红霉素，可使用氯霉素、利奈唑胺等低耐药率的抗生素。lmb和bca 是GBS的主要的毒力基因。
Abstract:
Objective To understandthe status of drug resistance and drug resistance genes and viru-lence genes of group B streptococcusin （GBS）Chaoyang district, Beijing. Methods Totally 29 strains of GBS were chosen from cervix of female subjects with pregnancy for 4 to 10 weeks in Bei-jing Chaoyang Maternal and Child Health Hospital from February to June in 2016. Broth microdilution method and disc diffusion method（Kirby-Bauer method）were used to determine the Bacterial resis-tance phenotype. Resistance genes and virulence genes of bacteria were detected by PCR. Results The sensitivity rate of GBS was100.0% to pennicillin, meropenem, cefotaxime, vancomycin, respec-tively. The drug resistance rate of GBS to erythromycin, tetracycline, levofloxacin, clindamycin, gati-floxacin, chloramphenicol, Linezolid was 82.76%,75.86%,44.83%,37.93%,37.93%,6.90%,6.90%, respectively. Penicillin resistant genes were found from 29 strains of GBS, including 28 strains of pbp1a （96.55%）, 26 strains of pbp2b（89.66%）, 19 strains of gyrB（65.52%）, 15 strains of linB （51.72%）, 14 strains of ermB（48.28%）, 13 strains of tetM （44.83%）, 11 strains of mefA （37.93%）, 8 strains of tetK（27.59%）, 5 strains of tetO（17.24%）. Totally 22 strains of toxingene such as lmb gene（75.86%）, 10 strains of ScpB gene, 13 strains of bca gene, 10 strains of hylB gene（34.48%）, 8 strains of bac gene（27.59%）were found in29 strains of GBS. Conclusion In Chaoyang district of Beijing, GBS has no resistant strains to penicillin, erythromycin is not recom-mended for patients with penicillin allergy, and antibiotics with low resistance rate such as chloramphen-icol and linezolid can be used. The lmb and bca are the main virulence genes of GBS.

参考文献/References:


[1] 马延敏,吴连方.B族链球菌对母婴健康的影响及诊断和防治[J]. 中华围产医学杂志,1998,1(2):109-111.
[2] 葛存兴,赵莹,王宏志,等.孕妇无乳链球菌感染及耐药性分析[J]. 中国现代药物应用,2016,10(12):205-206.
[3] 刘泽滨,聂署萍,陆学东,等.某院妇产科住院患者的无乳链球菌感染 情况及耐药性研究[J].检验医学与临床,2016,13(21):3049-3051.
[4] 杨旭峰.无乳链球菌和化脓性链球菌对氟喹诺酮类抗生素的耐药 性及耐药机制的研究[D].浙江大学,2013.
[5] 李丽.B族溶血链球菌基因型与新生儿侵袭感染临床型相关研究 [D].南华大学,2015.
[6] 李嫚.无乳链球菌和停乳链球菌的几种耐药基因对其毒力的影响 [D].内蒙古农业大学,2015.
[7] 赵丽琴.育龄妇女分离无乳链球菌对氟喹诺酮类抗生素的耐药性 及耐药机制研究[J].医学研究杂志,2015,44(8):149-151.
[8] 刘镜光,叶晓涛.深圳地区B族链球菌耐药基因流行研究[J].中华 医院感染学杂志,2017,27(1):54-56,81.
[9] 陈旭宾.围生期孕妇生殖道B族链球菌感染情况与耐药性研究[J]. 哈尔滨医药,2017,37(1):29-31.
[10] 刘丽,杨文东.围生期孕妇生殖道B族链球菌感染与耐药性分析 [J].现代临床医学,2016,42(4):264-266.
[11] 杨明,杨永弘,Dmitriev A,等.B族链球菌的红霉素耐药基因研究 [J].中华儿科杂志,2002,40(8):25-28.
[12] 申阿东,张桂荣,王咏红,等.B组链球菌的耐药性及红霉素耐药基 因检测研究[J].中华儿科杂志,2005,43(9):27-30.
[13] 卢赞.云南省昆明市产妇无乳链球菌携带率及耐药性分析[J].实 用医技杂志,2015,22(1):21-22.
[14] 张传飞.育龄妇女泌尿生殖道无乳链球菌感染情况与耐药性分析 [J].中国微生态学杂志,2016,28(9):1094-1096.
[15] 胡发龙.孕晚期妇女无乳链球菌感染与耐药性情况及对新生儿的 影响[J].中国微生态学杂志,2016,28(5):598-600.
[16] 苏锦珍,吴伟元,丁璐,等.围生期孕产妇感染或定植的B族链球菌 耐药情况、血清型、毒力基因及基因型分析[J].中华妇幼临床医学 杂志(电子版),2016,12(5):583-589.

备注/Memo

备注/Memo:
作者简介：杨琳，本科，主治医师，研究方向：计划生育 通信作者：宋衍燕，E-mail: songyanyan@cycdpc.org
更新日期/Last Update: 2019-06-28