|本期目录/Table of Contents|

[1]马红,魏芳,鲁华东,等.二甲双胍对非酒精性脂肪性肝病大鼠肝组织T-钙黏蛋白表达的影响[J].慢性病学杂志,2019,20(02):169-173.
　MA Hong*,WEI Fang,LU Hua-dong,et al.Effects of metformin on expressions of T-cadherin in the liver tissues of rats with non-alcoholic fatty liver disease[J].,2019,20(02):169-173.
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二甲双胍对非酒精性脂肪性肝病大鼠肝组织T-钙黏蛋白表达的影响(PDF)

《慢性病学杂志》[ISSN:1674-8166/CN:11-5900/R]

卷:
20
期数:
2019年02期
页码:
169-173
栏目:
论　著
出版日期:
2019-03-28

文章信息/Info

Title:
Effects of metformin on expressions of T-cadherin in the liver tissues of rats with non-alcoholic fatty liver disease
作者:
马红1;  魏芳1;  鲁华东2;  陈立刚3;  黄延玲1;  张文强1
厦门大学附属中山医院，福建医科大学厦门中山教学医院 （1. 内分泌科；2. 病理科；3. 消化内科），福建厦门361004
Author(s):
MA Hong*;  WEI Fang; LU Hua-dong;  CHEN Li-gang;  HUANG Yan-ling;  ZHANG Wen-qiang
*Department of Endocrinology，Zhongshan Hospital Xiamen University, Xiamen Zhongshan Teaching Hospital of Fujian Medical University，Xiamen，Fujian 361004，China Corresponding author：Ma Hong，Email：mah-169@163.com
关键词:
非酒精性脂肪性肝病; T-钙黏蛋白; 二甲双胍
Keywords:
Non-alcoholic fatty liver disease;  T-cadherin;  Metformin
分类号:
R541.4
DOI:
-
摘要:
目的探讨二甲双胍对非酒精性脂肪性肝病（NAFLD） 大鼠肝组织T-钙黏蛋白表达的影响，为临床研 究提供参考。方法雄性SD 大鼠40只随机分组，NC1组：8周正常饮食组；NC2组：16周正常饮食组；HFD1 组：8周高脂饮食组；HFD2 组：16周高脂饮食组；HFD+M 组：二甲双胍治疗组，即16周高脂饮食同时给予二 甲双胍治疗8 周。免疫组化法和实时荧光定量PCR 法检测各组大鼠肝组织T-钙黏蛋白的蛋白和mRNA 表达水 平。结果HFD+M 组大鼠肝组织的NAFLD 活动度评分（NAS） 较HFD2 组明显降低（P<0.01），T-钙黏蛋 白的蛋白和mRNA 表达亦明显下降（P<0.05）。肝组织T-钙黏蛋白表达的免疫组化评分与肝组织的炎症分级、 脂肪变百分比和气球样变程度均呈正相关（P<0.01）。结论二甲双胍可能通过下调肝组织T-钙黏蛋白的表达 发挥对NAFLD 的治疗作用。
Abstract:
Objective To investigate the hepatic expressions of T-cadherin and effects of metformin on expressions of T-cadherin in rats with non-alcoholic fatty liver disease （NAFLD） . Methods Forty male Sprague-Dawley rats were randomly divided into 5 groups:rats in NC1 group were fed a normal diet for eight weeks; rats in NC2 group were fed a normal diet for sixteen weeks; rats inHFD1 group were fed a high- fat diet for eight weeks;rats in HFD2 groupwere fed a high- fat diet for sixteen weeks; rats inHFD+M group were fed a high-fat diet for sixteen weeks and treated with metformin for eight weeks. T-cadherin protein expression levels in liver tissues were detected with immunohistochemistry, and the T-cadherin mRNA expression levels in livers were detected with fluorescent quantitative PCR. Results Compared with HFD2 group, NAFLD active score （NAS） of the liver tissue in the HFD+M group was significantly decreased （P<0.01） , and T-cadherinprotein and mRNA expression levels in HFD + M group were significantly decreased （P<0.05） . The immunohistochemistry scores of the T-cadherin in the liver tissues were positively related to the grade of inflammation, percentage of fat and ballooning degeneration （P<0.01） . Conclusion Metformin treatment alleviates the histological lesions of NAFLD and may play the therapeutic effect on NAFLD by down-regulating theexpression of T- cadherin in the liver tissue.

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备注/Memo

备注/Memo:
基金资助：福建省医学创新课题（2015-CXB-38） 作者简介：马红，博士，教授，研究方向：内分泌与代谢性疾病的诊治 通信作者：马红，E-mail: mah-169@163.com
更新日期/Last Update: 2019-02-28