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《慢性病学杂志》[ISSN:1674-8166/CN:11-5900/R]

卷:

期数:

2018年03期

页码:

254-258

栏目:

论著

出版日期:

2018-03-28

文章信息/Info

Title:

Clinical significance of serum miRNA let-7e and miRNA-375expression in the early diagnosis of esophageal adenocarcinoma

作者:

姜波¹; 文进¹; 刘全新¹; 高爱民¹; 赖建明¹; 毛仁贤¹; 赵胜乾²; 盛莹¹

解放军第159医院（1.病理科；2.消化内科），河南 驻马店 463000

Author(s):

JIANGBo * ; WENJin; LIUQuan-xin; GAOAi-min; LAIJian-ming; MAORen-xian; ZHAOSheng-qian; SHENGYing

*Departmentof Pathology, People'sLiberationArmy159Hospital,Zhumadian, Henan463000,China Correspondingauthor:SHENGYing,E-mail:jiang8848@163.com

关键词:

miRNA let-7e; miRNA-375; 食管腺癌; 早期诊断; 癌基因

Keywords:

miRNA let-7e;  miRNA-375;  Esophageal adenocarcinoma;  Early diagnosis;  Oncogene

分类号:

R735.1

DOI:

-

摘要:

目的 观察食管腺癌患者血清miRNA let-7e和miRNA-375表达及其临床意义。方法 选取2014年 1月—2017年3月来解放军第159医院就诊的食管腺癌患者65例作为食管腺癌组；选择同期行胃镜检查诊断为 Barrett食管的患者30例和健康体检者30例分别为Barrett食管组和健康对照组。用实时PCR的方法检测并比较各 组血清miRNA let-7e和miRNA-375表达，并观察食管癌患者血清miRNA let-7e和miRNA-375表达水平与 临床病理特征的关系。结果 食管腺癌组患者血清miRNA let-7e表达量明显高于Barrett食管组和健康对照组 （P<0.01），Barrett食管组高于健康对照组（P<0.01），手术后15d血清miRNA let-7e表达水平明显低于治疗前 （P<0.01）。食管腺癌组血清miRNA-375表达量明显低于Barrett食管组和健康对照组（P<0.01），而Barrett食 管组低于健康对照组（P<0.01），治疗后血清miRNA-375水平较治疗前明显升高（P<0.01）。食管腺癌组血清 miRNA let-7e和miRNA-375表达与性别、年龄、肿瘤部位、肿瘤大小和肿瘤浸润深度无明显相关性（P> 0.05），而与分化程度、淋巴转移和肿瘤分期具有明显相关性（P<0.01）。血清miRNA let-7e与miRNA-375表 达呈明显的负相关（r=－0.759，P<0.01）。结论 miRNA let-7e和miRNA-375基因参与了食管腺癌的发生 发展，miRNA let-7e促进肿瘤生长，miRNA-375抑制肿瘤生长，两组存在某种平衡，可能成为早期诊断食管 腺癌的生物标记物。

Abstract:

Objective To observe the expression of serum miRNA let-7e and miRNA-375 in patients with esophageal adenocarcinoma and its clinical significance.Methods Totally65 patients with esopha-geal adenocarcinoma, from January 2014 to March 2017, were selected as esophageal adenocarcinoma group; 30 patients with Barrett’s esophagus were selected for endoscopy as Barrett’s esophagus group; 30 healthy people were selected as healthy control group. The expression of serum miRNA let-7e and miR-NA-375was detected by real-time PCR. The levels of serum miRNA let-7e and miRNA-375were ob-served and compared in three groups, and the relationship between serum miRNA let-7e and miRNA-375 in esophageal adenocarcinoma patients and clinicopathological parameters were also observed. Results The level of serum miRNA let-7e in esophageal adenocarcinoma group was significantly higher than that in Barrett’s esophagus group and healthy control group（P<0.01）, and Barrett’s esophagus group was significantly higher than control group（P<0.01）. Serum miRNA let-7e was significantly decreased after treatment（P<0.01）. The expression of miRNA-375 in esophageal adenocarcinoma group was significant-ly lower than that in Barrett’s esophagus group and healthy control group（P<0.01）, and the expressionof Barrett’s esophagus was significantly lower than that of healthy control group（P<0.01）. After treat-ment, the serum miRNA-375 level was significantly higher than that before treatment （P<0.01）. The expressions of serum miRNA let-7e and miRNA-375 in esophageal adenocarcinoma patients were not significantly correlated with gender, age, tumor location, tumor size and tumor depth（P>0.05）, but they were significantly correlated with the degree of differentiation, lymphatic metastasis and tumor stag-ing （P<0.01）. The expression of serum miRNA let-7e in patients with esophageal adenocarcinoma was negatively correlated with the expression of serum miRNA-375（r=-0.759, P<0.01）. Conclusion The miRNA let-7e and miRNA-375 are involved in the development of esophageal carcinoma. miRNA let-7e promotes tumor growth and miRNA-375 inhibits tumor growth. There is some balance between the two makers, which may become biomarkers of early diagnosis of esophageal adenocarcinoma.

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备注/Memo

备注/Memo:

作者简介：姜波，本科，主治医师，主要从事消化道肿瘤的病理学诊断及相关技术检查工作 通信作者：盛莹，E-mail：jiang8848@163.com

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更新日期/Last Update: 2018-03-28