|本期目录/Table of Contents|

[1]刘银,王瑶,苏笮斌,等.中药复方养生茶对酒精性肝损伤大鼠的保护作用及其机制研究[J].慢性病学杂志,2018,(02):135-138.
　LIU Yin*,WANG Yao,SU Ze-bin,et al.Evaluation of protective effects and probable mechanism of herbal compound health tea against alcohol liver damages[J].,2018,(02):135-138.
点击复制

中药复方养生茶对酒精性肝损伤大鼠的保护作用及其机制研究(PDF)

《慢性病学杂志》[ISSN:1674-8166/CN:11-5900/R]

卷:
期数:
2018年02期
页码:
135-138
栏目:
论著
出版日期:
2018-02-01

文章信息/Info

Title:
Evaluation of protective effects and probable mechanism of herbal compound health tea against alcohol liver damages
作者:
刘银1;  王瑶1;  苏笮斌2;  许欣1
1. 四川大学华西公共卫生学院卫生检验与检疫系，成都610041； 2. 四川苏益康生物开发有限公司，成都610400
Author(s):
LIU Yin*;  WANG Yao;  SU Ze-bin; XU Xin
*Department of Health Inspection and Quarantine, West China School of Public Health, Sichuan University, Chengdu 610041, China Corresponding author: XU Xin, E-mail: 569810557@qq.com
关键词:
酒精性肝损伤; 中药复方养生茶; 药食同源; SD 大鼠
Keywords:
Alcohol liver damage;  Herbal compound health tea;  Affinal drug and diet;  SD rat
分类号:
R285.5
DOI:
-
摘要:
目的评价中药复方养生茶对酒精所致肝损伤的保护作用及其机制，为该中药复方的开发和应用提供科学 资料。方法将60只雌性SD大鼠随机分为三个剂量组[0.83 g/(kg · bw)、1.67 g/(kg · bw)、5.00 g/(kg · bw） ]， 以及空白组和模型组，连续30 d 按照2% （v/w） 容量灌胃，用50%乙醇以12 ml/(kg · bw)的剂量灌胃造成酒精 性急性肝损伤，全自动生化分析仪测定血清中天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、甘 油三酯（TG） 水平，生化法测定肝组织中氧化脂质（LPO）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶 （GSH）、丙二醛（MDA） 和甘油三酯（TG） 水平及病理检查。结果灌胃期间大鼠无不良反应。与模型组相 比，中药复方养生茶低剂量组ALT 水平降低（P<0.01），高剂量组GSH 水平有上升（P<0.05），三个剂量组的 LPO 浓度均下降（P<0.01），中剂量组（P<0.05）、高剂量组（P<0.01） MDA 水平降低，肝组织中脂滴含量降 低。结论中药复方养生茶可通过提高组织、细胞抗氧化能力，快速清除体内自由基，减轻其对肝组织的损 伤，减少血清脂质和肝脏脂质沉积，抑制脂类过氧化，从而缓解酒精性肝损伤。
Abstract:
Objective To evaluate the protective effects and probable mechanism of herbal compound health tea （HCHT） against alcohol liver damages of rat. Method Totally 60 female SD rats were randomly allocated to three HCHT groups [low dose group: 0.83g/ （kg · bw） , middle dose group: 1.67g/(kg · bw), high dose group: 5.00g/(kg · bw)], blank control group and model group. The HCHT groups were continuously received intragastric administration by 2% （v/w） for 30 days. Alcohol liver damages of rats were induced by intraperitoneal injection of alcohol with 12 ml/ （kg · bw） . Serum biochemical indexes （AST, ALT, TG） were detected by automatic biochemical analyzer. Several liver indexes [lipid peroxide （LPO） , malonyldialdehyde （MDA） , glutathione （GSH） , TG, superoxide dismutase （SOD） ] were examined, and pathological examination was carried out. Results Rats had no adverse reactions during gastric irrigation. Compared to the model control, ALT of low dose HCHT group was decreased （P<0.01） ; activity of GSH of high dose group was enhanced （P< 0.05） ; activities of LPO of three HCHT groups were decreased （P<0.01） ; MDA levels and content distribution of membranous lipid droplet of middle dose group and high dose groups were decreased （P<0.05 or P<0.01） . Conclusion This HCHT can improve cell antioxidant capacity, remove freeradicals, reduce the damage in liver, reduce serum lipid and liver lipid deposition, inhibition of lipid peroxidation, and thus relieve the alcoholic liver damage.

参考文献/References:

[1] 邱萍,李相,孔德松,等.酒精性肝病发病机制研究的新进展[J].中 国药理学通报,2014,30(2):160-163.
[2] 刘睿,任金威,陈启贺,等. 人参低聚肽对急性酒精性肝损伤大鼠 的保护作用[J].现代预防医学,2016,43(15):2820-2824.
[3] 田洁,柯翔鸿,田辉,等. 茯苓女贞子复合胶囊对酒精性肝损伤保 护作用的实验研究[J]. 公共卫生与预防医学,2012,23(4):106- 107.
[4] 中华医学会肝病学分会脂肪肝和酒精性肝病学组.酒精性肝病诊 疗指南[J].胃肠病学,2010,15(10):4-5.
[5] Shukla SD, Pruett SB, Gyongyi S, et al. Binge Ethanol and Liver: New Molecular Developments [J]. Alcohol Clin Exp Res, 2013,37(4):550-557.
[6] 周春晖,苏烨,李凤芝.金银花提取物对小鼠化学性肝损伤的保护 作用初步研究[J].现代食品科技,2010,26(4):351-353.
[7] Bhattacharya A, Ghosal S, Bhattacharya SK. Anti- oxidant effect of Withania somnifera, glycowithanolides in chronic footshock stress- induced perturbations of oxidative free radical scavenging enzymes and lipid peroxidation in rat frontal cortex and striatum [J]. J Ethnopharmacol, 2001, 74(1):1-6.
[8] Udoh US, Valcin JA, Gamble KL, et al. The Molecular Circadian Clock and Alcohol-Induced Liver Injury [J]. Biomol Ther (Seoul), 2015,5(4):2504-2537.
[9] Jia R, Cao LP, Du JL, et al. Effects of carbon tetrachloride on oxidative stress, inflammatory response and hepatocyte apoptosis in common carp (Cyprinus carpio) [J]. Aquat Toxicol, 2014,152(152C):11-19.

备注/Memo

备注/Memo:
作者简介：刘银，硕士研究生，研究方向：微生物/公众健康与检验 通讯作者：许欣，E-mail:569810557@qq.com
更新日期/Last Update: 2018-02-28