|本期目录/Table of Contents|

[1]孙建伟,陈瑶,刘青青,等.MDR1基因多态性与乳腺癌的相关性研究[J].慢性病学杂志,2016,(01):48-51.
　SUN Jian-wei,CHEN Yao,LIU Qing-qing,et al.Relationship between MDR1gene polymorphism and breast carcinoma[J].,2016,(01):48-51.
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MDR1基因多态性与乳腺癌的相关性研究(PDF)

《慢性病学杂志》[ISSN:1674-8166/CN:11-5900/R]

卷:
期数:
2016年01期
页码:
48-51
栏目:
论著
出版日期:
2016-02-15

文章信息/Info

Title:
Relationship between MDR1gene polymorphism and breast carcinoma
作者:
孙建伟1; 陈瑶2; 刘青青2; 贾玲1; 陈居敏1; 刘春生1; 纳志明1
云南省第一人民医院/昆明理工大学附属医院（1.乳腺甲状腺肿瘤科；2.药学科），昆明 650034
Author(s):
SUN Jian-wei; CHEN Yao; LIU Qing-qing; JIA Ling; CHEN Ju-min; LIU Chun-sheng; NA Zhi-ming
FirstPeople’s HospitalofYunnanProvince,Kunming,Yunnan650034, China Correspondingauthor:SUNJian-wei,E-mail:sunjianwei0630@163.com
关键词:
乳腺肿瘤; 多药耐药基因1; 多态性
Keywords:
Breast cancer;  MDR1;  Polymorphisms
分类号:
R737.9
DOI:
-
摘要:
目的 探讨MDR1基因多态性与其在乳腺癌患者癌组织中表达水平、药物治疗反应及预后关系。方 法 选取153例乳腺癌组织及40例配对癌旁组织、20例乳腺纤维腺瘤组织，利用PCR-RFLP技术检测 MDR1 exon12(1236)、exon21(2677)、exon26(3435) 3 个位点的多态性，以RT-qPCR 技术对MDR1 mRNA进行相对定量，分析不同基因型患者对药物治疗的反应及预后。结果 153例乳腺癌病例中有65例 有MDR1基因扩增，总体扩增率为42.5%，表达值范围0~1.42×10 5基因拷贝/μl。在13例有效病例中，基 因型CC型占11例、GT型占2例，而TT型则全部无效，TT型与其他型差异有统计学意义（P= 0.031 7），其中C3435T的TT化疗疗效显著低于CC和GT型（P=0.006 5）。结论 MDR1基因在乳腺癌 组织中的高表达，其SNP位点基因型可作为药物治疗反应及预后的预测因子。
Abstract:
Objective To investigate the relationship between MDR1 gene polymorphism, response to drug treatment and prognosis of breast carcinoma patients. Methods A total of153 cases of breast can-cer tissues, 40 cases matched adjacent tissues, and 20 cases of breast fibroadenoma tissue were studied. PCR-RFLP technique was used to detect MDR1 exon12 (1236), exon21 (2677), exon26 (3435), 3 sin-gle nucleotide polymorphic (SNP) loci. RT-qPCR technique was used to detect relative expression level of MDR1 mRNA. Based on the results, response to drug treatment and prognosis of different geno-types were compared. Results Among the153 cases of breast cancer, MDR1 gene amplification was successful in 65 cases, with an overall amplification rate of 42.5%. Expression values ranged from0 to 1.42×10 5 gene copies/μl. Among the 13 cases of effective cases, genotype CC accounted for 11 cases, GT accounted for2 cases. Genotype TT were all invalid. There is a significant difference between the TT and other types (P=0.031 7). The chemotherapy effectiveness in the C3 435T type is signifi-cantly lower than those in the CC and GT type (P=0.006 5). Conclusions The expression level of MDR1 gene in breast cancer, and the gene polymorphism can be predictors of drug response and prog-nosis in chemotherapy treatment.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介：孙建伟，硕士，副主任医师，研究方向：乳 腺肿瘤的基础与临床研究 通信作者：孙建伟，E-mail: sunjianwei0630@163.com
更新日期/Last Update: 2016-03-18