|本期目录/Table of Contents|

[1]钟亚莉,李健,赵治国,等.PDHA1 基因敲除对人食管癌细胞生物学性状影响[J].慢性病学杂志,2015,(05):532-534.
　ZHONG Ya-li *,LIJian,ZHAO Zhi-guo,et al.Effect of PDHA1gene knockout on biological phenotypes of esophageal cancer cell line[J].,2015,(05):532-534.
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PDHA1 基因敲除对人食管癌细胞生物学性状影响(PDF)

《慢性病学杂志》[ISSN:1674-8166/CN:11-5900/R]

卷:
期数:
2015年05期
页码:
532-534
栏目:
论著
出版日期:
2015-10-15

文章信息/Info

Title:
Effect of PDHA1gene knockout on biological phenotypes of esophageal cancer cell line
作者:
钟亚莉1; 李健2; 赵治国1; 索振河3
1.郑州大学第二附属医院消化内科，郑州 450017；2.河南省人民医院消化内科，郑州 450003； 3.郑州大学第一医附属医院肿瘤内科，郑州 450052
Author(s):
ZHONG Ya-li * ; LIJian; ZHAO Zhi-guo; SUO Zhen-he
*DepartmentofDigestiveDiseases, theSecondAffiliated HospitalofZhengzhou Univerrsity,Zhengzhou,Henan450017,China Correspondingauthor:SUOZhen-he, E-mail:zhenhe.suo@medisin.uio.no
关键词:
PDHA1; 食管癌; 转移
Keywords:
PDHA1;  Esophageal cancer;  Metastasis
分类号:
R735.1
DOI:
-
摘要:
目的 研究PDHA1基因敲除对人食管癌细胞的影响，以了解PDHA1基因表达下降在人食管癌复发 转移中的作用。方法 采用细胞转染TALEN质粒对的方法，利用食管癌细胞系EC9706构建PDHA1基 因敲除细胞株，比较其与亲本细胞在表型差别。结果 PDHA1基因敲除后，PDHA1蛋白表达水平明显下 降，细胞表现出生长速度减慢；但在体外细胞移动实验中表现出穿膜能力明显增强，穿过滤膜的细胞数 （45±8）个，而亲本细胞仅有（24±6）个，差异有统计学意义（P<0.05)。结论 成功建立PDHA1基因敲 除的稳定细胞系，PDHA1基因敲除后食管癌细胞增殖受到抑制，但是细胞体外运动能力增强，提示 PDHA1基因表达下降可能在人食管癌复发、侵袭转移中有重要的意义。
Abstract:
Objective To observe the effect of PDHA1 gene knockout on the esophageal cancer cell lines, in order to analyze Warburg effect in malignancy development of human esophageal cancer. Meth-odsWe developed the PDHA1 gene knockout cell line and then investigated the phenotypic changes of EC9706. ResultsWith PDHA1 gene knockout, the PDHA1 protein expression downregulated and the cell exhibited slower proliferation. In the transwell assay, migrated cell number of PDHA1KO cell and parental cell number were 45±8 cells and 24±6 cells (P<0.05) respectively. ConclusionsPDHA1 gene knockout cell line has been established. The proliferation of PDHA1KO cell was inhibited. However, cell migration ability enhanced. Our study indicates that downregulated or deleted PDHA1 may contrib-ute to relapse and metastasis of human esophagus cancer.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金面上项目（81272824） 作者简介：钟亚莉，硕士研究生，研究方向：代谢与肿 瘤 通信作者：索振河，E-mail: zhenhe.suo@medisin.uio.no
更新日期/Last Update: 2015-10-16