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《慢性病学杂志》[ISSN:1674-8166/CN:11-5900/R]

卷:

期数:

2015年05期

页码:

494-496

栏目:

论著

出版日期:

2015-10-15

文章信息/Info

Title:

Changes of mRNA expression related with generation and maturation of mirna in workers exposed to arsenic

作者:

文卫华; 成会荣; 王攀; 李瑛

云南省疾病预防控制中心，昆明 650022

Author(s):

WEN Wei-hua; CHENG HUI-rong; WANG Pan; LIYing

YunnanCenterforDisease Prevention andControl,Kunming650022,China Correspondingauthor:LiYing E-mail:dongsijiehua@sina.com

关键词:

砷;  miRNA; 相关mRNA

Keywords:

Arsenic;  miRNA ;  relative mRNA

分类号:

R542.2

DOI:

-

摘要:

目的 探讨职业砷接触工人miRNA生成和成熟相关mRNA表达变化规律，深入认识砷的致癌机制。方 法 整群随机抽取正在进行砷冶炼的工人43名作为高暴露组，已经停止砷冶炼85d的工人36名作为低暴露 组，对照组24人。用实时荧光定量PCR（RT-PCR）检测外周血miRNA生成和成熟相关mRNA表达情况，采 用氢化物发生原子吸收分光光度法检测尿砷含量。尿砷浓度和相关mRNA的-△△Ct值组间比较采用t检 验。检验水准α＝0.05。结果 高暴露组尿砷为（1738.62±1361.62）μg/g肌酐，低暴露组为（561.20±451.50）μg/ g肌酐，对照组为（17.63±11.07）μg/g肌酐，各组间差异具有统计学意义(P<0.01)。高暴露组外周血miRNA生 成和成熟相关mRNA Lin28、Lin28b、Exportin-5、Trbp、Dicer、Ago2 RT-PCR扩增-△△Ct与低暴露组和对 照组间差异均具有统计学意义（P＜0.01），而低暴露组与对照组间差异均无统计学意义(P＞0.05)。结论 砷冶 炼工人机体内可能存在大量miRNA生成和成熟相关mRNA表达增加。此过程是可逆的,砷中毒患者早诊、 早治非常重要。

Abstract:

Objective To investigate the rule of mRNA expression related with generation and matura-tion of miRNA in workers exposed to arsenic, study the carcinogenic mechanism of arsenic deeply. Methods We selected43 workers in one arsenic plant which was producing arsenic trioxide (high expo-sure group), and 36 workers from another plant which the production had stopped85 days (low expo-sure group) by random cluster sampling. RT-PCR was performed to detect the expressions of mRNAs related with generation and maturation of miRNA. It was student's t test used to analyse the differences along groups for urinary arsenic and the -△△Ct value of relative miRNAs. Test level wasα＝0.05. Results The concentration of urinary arsenic were（1738.62±1361.62）μg/g Cr in high exposed group， （561.20±451.50）μg/g Cr in low exposed group, and（17.63±11.07）μg/g Cr in control group, and there were significant difference along three group（P＜0.01). The -△△Ct came from RT-PCR for Lin28, Lin28b,Exportin-5, Trbp, Dicer and Ago2 were（9.88±2.42）,（8.49±3.10）,（9.57±2.36）,（6.07±1.62）, （6.96±1.60）and（6.91±2.02）respectively in mRNAs（P＜0.01), but not between low exposed group and control group for all mRNAs（P＞0.05). Conclusions There are many miRNAs which are generating and maturating in workers produce arsenic trioxide. At the same time, the expressions of many relativemRNA increase. The process is reversible, so it is of great important to diagnosis and treat ear-ly patients.

参考文献/References:

[1] Reichard JF, enburger MS, Puga A. Long term low-dose ar? senic exposure induces loss of DNA methylation［J］. Bio? chem Biophys Res Commun,2007,352(1):188–192.
[2］ Wen WH, Wen JH, Lu L,et al. Metabolites of arsenic and in? creased DNA damage of p53gene in arsenic plant workers ［J］.ToxicolApplPharmacol,2011,254(1):41-47.
[3］ Winter J, Jung S, Keller S,et al. Many roads to maturity: mi? croRNA biogenesis pathways and their regulation［J］. Nat. cellbiol,2009,11(3):228-234.
[4］ Weihua Wen, Wangjun Che, Lin Lu,et al. Increased damage of exon5of p53gene in workers from an arsenic plant［J］． Mut, Res,2008,643(1-2):36-40.
[5］ Yamauchi H. Evaluation of DNA damage in patients with arse? nic poisoning: urinary8-hydroxydeoxyguanine. Toxicol. Appl ［J］.Pharmacol,2004,198(3):291-296.
[6］ Huang HY, Hopper AK. In vivo biochemical analyses reveal distinct roles ofβ-importins and eEF1A in tRNA subcellular traffic［J］.Genes Dev,2015,29(7):772-83.
[7］ 曹文明,高赟,杨红健,等. Dicer1基因单核苷酸多态性与浙江 地区BRCA1/BRCA2阴性家族性乳腺癌的相关性研究［J］． 肿瘤学杂志,2013,19(5):364-368.
[8］ 申红红,牛昀.Lin28的功能研究进展［J］.中南大学学报(医学 版),2014,39(9):964-968.

备注/Memo

备注/Memo:

基金项目：国家自然科学基金资助项目（81160343和30860238）；云南省应用基础研究项目（2011B147）；云南省高层次 卫生技术人才培养基金（D-201250） 作者简介：文卫华，博士，主要从事职业卫生与中毒控制工作 通信作者：李瑛，E-mail：dongsijiehua@sina.com

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更新日期/Last Update: 2015-10-16