|本期目录/Table of Contents|

[1]乔羽,解颖,高恩宇,等.参苈加颗粒对慢性心衰大鼠心肌EG-VEGF 表达的影响[J].慢性病学杂志,2014,(06):431-433.
　QIAO Yu,XIE Ying,GAO En-yu,et al.The effect of SHENLIJIA Granule on EG-VEGF expression in heart failure rats screened by the protein chips[J].,2014,(06):431-433.
点击复制

参苈加颗粒对慢性心衰大鼠心肌EG-VEGF 表达的影响(PDF)

《慢性病学杂志》[ISSN:1674-8166/CN:11-5900/R]

卷:
期数:
2014年06期
页码:
431-433
栏目:
论著
出版日期:
2014-07-30

文章信息/Info

Title:
The effect of SHENLIJIA Granule on EG-VEGF expression in heart failure rats screened by the protein chips
作者:
乔羽;  解颖;  高恩宇;  陈飞;  李富震;  柳成刚;  刘春红;  常佳怡;  姜德友
黑龙江中医药大学基础医学院伤寒教研室，黑龙江哈尔滨150040
Author(s):
QIAO Yu;  XIE Ying;  GAO En-yu;  CHEN Fei;  LI Fu-zhen;  LIU Cheng-gang;  LIU Chun-hong;  CHANG Jia-yi;  JIANG De-you
Heilongjiang University of Chinese Medicine, Harbin，Heilongjiang 150040, China Corresponding author:JIANG De-you,E-mail:jiangdeyou@126.com
关键词:
蛋白芯片; 参苈加颗粒; 心力衰竭; 内分泌腺来源的血管内皮生长因子
Keywords:
Protein chips; SHENLIJIA Granule;  Heart failure; Endocrine gland-derived vascular endothelial growth factor (EG-VEGF)
分类号:
R541.6
DOI:
-
摘要:
目的应用蛋白芯片技术筛选并分析参苈加颗粒对慢性心力衰竭（以下简称心衰） 大鼠心肌内分泌腺 源性血管内皮生长因子（EG-VEGF） 表达的影响。方法从70 只清洁级Wistar 大鼠中随机抽取15 只作 为空白组（仅给予生理盐水），其余55 只用阿霉素制作心衰模型。6 周后，将造模成功的45 只大鼠随机分 为治疗组（25 只） 和模型组（20 只），分别给予参苈加颗粒和生理盐水灌胃，1 次/d。4 周后取大鼠左心室 组织，用蛋白芯片技术分析实验大鼠标本EG-VEGF 的表达。结果与空白组比较，模型组EG-VEGF 的表达下调，相对丰度比为0.52；与模型组比较，治疗组EG-VEGF 的表达上调，相对丰度比为0.66；治 疗组与空白组相比表达无差异，相对丰度比为0.79。结论参苈加颗粒可通过调节EG-VEGF 的表达，改 善心肌纤维化程度，对心衰大鼠有一定的保护作用。
Abstract:
Objective To assess the effect of SHENLIJIA Granule on EG- VEGF expression in heart failure rats by the protein chips. Methods There were 70 Wistar rats in this experiment. Fifteen rats were randomly selected as blank group, the others were induced by intraperitoneal injection of doxorubicin. The survival rats were randomly divided into model and treatment group. After the model establishment. In the meanwhile, rats in the treatment group were treated with gastric perfusion of Chinese herb SHENLIJIA Granule, once a day for twenty days. And those in the blank and model groups were treated with gastric perfusion saline. Four weeks later, gathering the left cardiac muscular tissue for the protein chips. Results Compared with the blank group, the EG-VEGF expression in the model group was down and their relative abundance was 0.52. Compared with the model group, the EG-VEGF expression in the treatment group was up and their relative abundance was 0.66. There was no difference between the treatment group and the blank group, their relative abundance was 0.79. Conclusions SHENLIJIA Granule improved the degrees of myocardium fibrosis and protected the heart by raising the expression of EG-VEGF.

参考文献/References:

[1] 李雨真,朱栋栋,李洁,等.补肾活血汤对慢性心力衰竭大鼠血清 sFas、MMP-2 的影响［J］ .世界中西医结合杂志,2012,7(5): 394.
[2］ 马文东,李曙光,叶再元.蛋白质组学技术及胃癌蛋白质组学研 究进展［J］ .现代中西医结合杂志,2008,17(15):2399.
[3］ Lin R, LeCouter J, Kowalski J, et al. Characterization of en? docrine gland- derived vascular endothelial growth factor sig? naling in adrenal cortex capillary endothelial cells ［J］ . Biol Chem, 2002,277(10):8724-8729.
[4］ Kisliouk T, Podlovni H, Meidan R. Unique expression and regulatory mechanisms of EG- VEGF/prokineticin- 1 and its receptors in the corpus luteum ［J］ . Ann Anat, 2005,187(5): 529-537.
[5］ LeCouter J, Kowalski J, Foster J, et al. Identification of an an? giogenic mitogen selective for endocrine gland endothelium ［J］ . Nature, 2001,412(6850):877-884.
[6］ Thomas M, Keramidas M, Monchaux E, et al. Role of adreno? corticotropic hormone in the development and maintenance of the adrenal cortical vasculature ［J］ . Microsc Res Tech, 2003,61(3):247-251.radation in the Hulth-Telhag model of osteoarthritis ［J］．Os? teoarthr Cartil, 1999,7( 6):539-547.
[2］ 徐英杰,尹羽薇,董清平.中药超微粉贴敷干预对兔骨性关节炎 关节软骨中MMP- 1、TIMP- 1 表达影响的实验研究［J］． 中医药信息,2011,28(4):134-136.
[3］ 苗明三.实验动物和动物实验技术［M］．北京:中国中医药出版 社出版,1997:143.
[4］ Pelletier JP， LasCau- Comman V，Jovanovico，et al.Selec? tive inhibition of inducible nitric oxide Synthase in experimental oateoarthritis is associated with reduction in tissue levels of catadbolic factors ［J］．J Rheumatol, 1999(26):2002-2014.
[5］ Fu X, Parks WC, Heinecke JW. Activation and silencing of matrix metalloproteinases ［J］．Semin Cell Dev Biol, 2008,19 (1):2-13.
[6］ Malemud CJ, Goldberg VM. Future directions for search and treatment of osteoarthritis ［J］． Front Biosci, 1999(4):0762- 0771.
[7］ Osch GJ, Kraan PM, Valburg AA, et al. The relation between cartilage damage and osteophyte size in a murine model for os? teoarthritis in the knee ［J］．Rheumatol Int, 2006,16(5):115- 119.
[8］ Close DR. Matrix metalloproteinase inhibitors in rheumatic dis? eases ［J］．Ann Rheum Dis, 2001,60(Suppl 3):62-67.
[9］ 章昀.基质金属蛋白酶与神经系统疾病［J］．国外医学:神经病 学神经外科学分册,2002,29(02):140-143.
[10］ 田华,党耕町,娄思权,等.基质金属蛋白酶及其抑制剂在软骨细 胞中的表达及其意义［J］．北京医科大学学报,1999,31(02): 189.
[11］ 郭常安,陈峥嵘,张秀荣.骨性关节炎基质金属蛋白酶1，2，3 及 其抑制物的表达与软骨退变的关系［J］．复旦学报:医学版, 2002,29(04):244.
[12］ Flannery CR, Little CB, Catersou B, et al. Effects of culture conditions and exposure to catabolic stimulators(IL- 1 and reti? uoic acid) on the expression of matrix metalloproteiuases (MMPs) and disintegrin metalloproteinases (ADAMs) by artic? ular cartilage choudrocytes ［J］． Matrix Biol, 1999,18( 3): 225-237.

备注/Memo

备注/Memo:
基金项目：哈尔滨市科技创新人才研究专项资金项目资助（2009RFXXS007） 作者简介：乔羽，博士，讲师，主要从事经方治疗外感与疑难杂病的临床与基础研究 通信作者：姜德友，E-mail: jiangdeyou@126.com · 431 ·
更新日期/Last Update: 2014-07-30