|本期目录/Table of Contents|

[1]马宁,张盼红,李阳,等.氯乙烯染毒对大鼠肝细胞P16、CCND1和CDK4基因mRNA表达的影响[J].慢性病学杂志,2013,(04):252-254.
　MA Ning*,ZHANG Pan-hong,LI Yang,et al.P16,CCND1 and CDK4 mRNA expression in hepatocytes of rats exposed to vinyl chloride monomer[J].,2013,(04):252-254.
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氯乙烯染毒对大鼠肝细胞P16、CCND1和CDK4基因mRNA表达的影响(PDF)

《慢性病学杂志》[ISSN:1674-8166/CN:11-5900/R]

卷:
期数:
2013年04期
页码:
252-254
栏目:
论著
出版日期:
2013-04-30

文章信息/Info

Title:
P16,CCND1 and CDK4 mRNA expression in hepatocytes of rats exposed to vinyl chloride monomer
作者:
马宁; 张盼红; 李阳; 王慧; 高怡; 仇玉兰
山西医科大学公共卫生学院卫生毒理学教研室，山西 太原030001
Author(s):
MA Ning*; ZHANG Pan-hong; LI Yang; WANG Hui; GAO Yi; QIU Yu-lan
*Department of Toxicology,School of Public Health,Shanxi Medical University, Taiyuan,Shanxi 030001,China Corresponding author:QIU Yu-lan,E-mail:ylqiu@sina.com
关键词:
氯乙烯; P16; CCND1; CDK4; mRNA表达
Keywords:
Vinyl chloride monomer;  P16;  CCND1;  CDK4;  mRNA expression
分类号:
R14
DOI:
-
摘要:
目的通过检测氯乙烯(VCM）对大鼠肝脏细胞P16、CCND1和CDK4基因mRNA表达的变化，从细胞周期调控角度，探讨VCM可能的致癌机制。方法选用健康SD大鼠32只，按体重随机分为4组：阴性对照组，5、25、125 mg/kg氯乙烯染毒组。采用腹腔注射染毒，隔日染毒，每周3次，连续6周。采用实时荧光定量PCR方法，测定大鼠肝脏细胞P16、CCND1和CDK4基因mRNA表达量。结果氯乙烯对大鼠肝脏细胞CCND1和CDK4基因mRNA表达量随着染毒剂量的升高而降低，且125 mg/kg染毒组与对照组比较，差异均有统计学意义(P<005)。P16 基因mRNA表达量没有明显改变，差异无统计学意义。结论VCM对大鼠肝脏细胞周期的影响可能是通过下调CCND1和CDK4基因mRNA的表达。
Abstract:
Objective Detect the change of P16,CCND1 and CDK4 gene mRNA expression in rat liver cells,from the field of cell cycle regulation,to explore the possible carcinogenic mechanism. Methods Thirty-two Sprague Dawley(SD)rats(half male and half female)were randomly divided into negative control group and three experimental groups exposed to 5,25,and 125mg/kg VCM.The rats were treated with clean air and different doses VCM by intraperitoneal injection with three times a week for 6 weeks,and rats were killed at the end of 6 weeks.Real-time quantitative PCR method was applied to detect the change of P16,CCND1 and CDK4 gene mRNA expression in liver cells. Results Compared with the control,CCND1 and CDK4 gene mRNA expression in liver cells of 125 mg/kg dose group decreased significantly(P<0.05).P16 gene mRNA expression did not change significantly. Conclusions VCM affects cell cycle through decreasing CCND1 and CDK4 gene mRNA expression.

参考文献/References:

[1] IARC.Vinyl chloride,chloroethene A.Evidence for carcinogenicity to humans(sufficient)vinyl chloride has been associated with tumours of the liver,brain,lung and haematolymphopoietic.IARC,1987,(7):373.
[2] 朱守民,王爱红,夏昭林,等.氯乙烯致大鼠DNA损伤与DNA损伤修复酶和p53蛋白的表达[J].工业卫生与职业病,2005,31(6):353- 356.
[3] Zhu S-M,Xia Z-L,Wang A-H,et al.Polymorphisms and haplotypes of DNA repair and xenobiotic metabolism genes and risk of DNA damage in Chinese vinyl chloride monomer(VCM)-exposed workers [J].Toxicol Lett 2008,178(2):88-94.
[4] Lei Y-C,Yang H-T,Ma Y-C,et al.DNA single strand breaks in peripheral lymphocytes associated with urinary thiodiglycolic acid levels in polyvinyl chloride workers [J].Mutat Res 2004,561(1-2):119-126.
[5] 李阳,王慧,仇玉兰,等.氯乙烯对大鼠肝细胞细胞周期及凋亡影响 [J].中国公共卫生,2012,28:81-83.
[6] 亓明.CDK2、CDK4基因在大鼠自体移植静脉中的表达和意义[D].中国医科大学,2004.
[7] Thoms HC,Dunlop MG,Stark LA.p38-mediated inactivation of cyclin D1/cyclin-dependent kinase 4 stimulates nucleolar translocation of RelA and apoptosis in colorectal cancer cells [J].Cancer Res,2007,67(4):1660-1669.
[8] Miyakawa Y,Matsushime H.Rapid downregulation of Cyclin D1 mRNA and protein levels by ultraviolet irradiation in murine macrophage cells [J].Biochem Biophys Res Commun,2001,284(1):71-76.
[9] Viallard JF,Lacombe F,Belloc F,et al.Molecular mechanisms controlling the cell cycle:Fundamental aspects and implications for oncology [J].Cancer Radiother,2001,5(2):109-129.

备注/Memo

备注/Memo:
基金项目:中国博士后科学基金(20100471583) 作者简介:马宁,硕士研究生,主要从事职业毒理学研究 通讯作者:仇玉兰,E-mail:ylqiu@sina.com
更新日期/Last Update: 2013-05-20